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アクティブボード・２００２年 ５月
研究発表を行った学会；第２４回日本分子生物学会年会
２００１年１２月９日～１２日（横浜）
タイトル；Involvement of Oct-3/4 in SDIA-mediated neurogenesis of ES cells
発表者；下崎 康治 氏
（発生医学研究センター 転写制御分野）
Abstract；
Oct-3/4, which belongs to the POU transcription factor superfamily, plays a critical role in maintaining pluripotency of embryonic stem cells. It has been recently reported that neurogenesis of ES cells is dramatically promoted by SDIA (for stromal cell-derived inducing activity), which accumulates on the surface of a stromal cell line PA6. We here demonstrate that Oct-3/4, a master regulator of ES cell pluripotency, also plays a critical role in the process of SDIA-mediated neurogenesis of ES cells. The expression of Oct-3/4 was found to be sustained in ES cells cultured with SDIA but downregulated when SDIA was omitted. Furthermore, when Oct-3/4 was continuously expressed in ES cells using a tetracycline-regulated expression system, efficient neuronal differentiation in serum-free conditions was observed even in the absence of SDIA. The continuous expression of Oct-3/4 accelerated the differentiation of ES cells to primitive ectoderm and promoted the appearance of cells positive for Nestin, a neural stem/precursor cell marker. Moreover, SDIA-mediated neurogenesis of ES cells was enhanced by the continuous Oct-3/4 expression. Taken together, it is conceivable that Oct-3/4 functions as a key regulator in the primitive neural stem cell formation and the SDIA-mediated neurogenesis of ES cells.
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