|
アクティブボード・２００３年３月
研究発表を行った学会；Annual Meeting of Society for Neuroscience
２００２年１１月２日～７日（Orlando, Florida）
タイトル；Expression and the possible function of SSEA-1 in the developing brain.
発表者；柳澤 亮 氏
（熊本大学・発生医学研究センター・転写制御分野）
Abstract；
Neuronal and glial cells organizing the central nervous system are generated from common neural precursor cells during development. We found a carbohydrate antigen, stage-specific embryonic antigen-1 (SSEA-1), was expressed in the lateral wall of lateral ventricles of the embryonic mouse telencephalon where neural precursor cells are abundantly present. SSEA-1 immunoreactivity was co-localized with a marker of neural precursor cells, nestin, and its intensity was decreased in accordance with the progress of development. SSEA-1 was also expressed in neuroepithelial cells cultured with basic fibroblast growth factor, but was missing in the mature neurons and astrocytes. Neuroepithelial cells were known to form aggregates of neural precursor cells called neurospheres on the non-adhesive substratum, from which cells tend to migrate when transferred on adhesive substratum. We found that a monoclonal antibody AK97 which cross-reacts with SSEA-1 negatively regulated the migration of the cells from neurospheres on fibronectin-coated dish, probably by interfering with the interaction between integrin and fibronectin by binding to SSEA-1 on carbohydrate chains of the beta1 integrin molecule. Taken together, SSEA-1 is suggested to be expressed in immature neural cells and involved in their migration in the developing brain.
|