研究発表を行った学会；第２７回日本分子生物学会年会
２００４年１２月　８日〜１２月１１日（神戸）
and
American Heart Association, Scientific Sessions 2004,
2004.11.7-11 (New Orleans, USA).
タイトル；α/β Hydrolase Domain Containing 2 Suppresses Smooth Muscle Cells Migration and Inhibits Development of Experimental Intimal Hyperplasia.
発表者；宮田　敬士　氏
　　　（熊本大学　発生医学研究センター　臓器形成分野）
Abstract；
　To search for proteins that play a role in development of vascular smooth muscle cells (SMCs), we have been using the exchangeable gene trap method. One of these trap ES clones, termed as Ayu8025, showed specific expression of marker gene in SMCs of adult mice. The gene trapped is shown to be Abhd2 (alpha/beta hydrolase domain containing 2) protein, whose expression and function has not been elucidated. We found that Abhd2 gene was expressed in SMCs of vascular system, bronchial tract, elementary duct and uterus in adult. To analyze the role of Abhd2 in vascular wall, we examined the intimal hyperplasia response induced vascular injury and performed SMC explants assays. Abhd2 deficiency mice showed the marked thickened layer of neointimal hyperplasia after cuff-induced surgery. Arterial explants SMC form the aorta of Abhd2 deficiency mice showed that the SMCs migration was promoted in comparison with wild type mice. The chemoattractant-derived migration through a reconstituted basement membrane barrier was significantly promoted in cultured SMCs derived from Abhd2 deficiency mice, although no difference was observed in SMCs proliferative response between wild type and Abhd2 deficiency mice. These results suggest that Abhd2 suppresses SMCs migration and inhibits development of experimental intimal hyperplasia, and that Abhd2 might play an important role in stress response.