熊本大学・遺伝子実験施設
熊本市本荘２−２−１
Tel : (096) 373-6501, FAX : (096)373-6502
２００３年 ５月１６日 更新
T3 や T7 など、よく使われるプライマーを大量合成し、小分けしたものです。濃度１００ピコモル／マイクロリットルで、１００マイクロリットルずつ分注してあります（Total 10 nmol）。ＰＣＲ用です。sequence反応に使用出来るかどうかのチェックは行っていません。１本７００円です。
支払方法については、原則として、機械を利用する場合の利用者負担金と同様に、年１回集計し、移算手続きを行うことにします。
| No. | Name | Size | Sequence | Note |
|---|---|---|---|---|
| 1 | T3 | 21 mer | 5'-att aac cct cac taa agg gaa-3' | pBluescript II |
| 2 | T7 | 20 mer | 5'-taa tac gac tca cta tag gg-3' | pBluescript II |
| 3 | M13-F | 20 mer | 5'-gtt gta aaa cga cgg cca gt-3' | M13 or pUC |
| 4 | M13-R | 21 mer | 5'-agg aaa cag cta tga cca tga-3' | M13 or pUC |
| 5 | λ11-F | 24 mer | 5'-ggt ggc gac gac tcc tgg agc ccg-3' | λgt11 |
| 6 | λ11-R | 24 mer | 5'-ttg aca cca gac caa ctg gta atg-3' | λgt11 |
| 7 | λ10-F | 20 mer | 5'-gct ggg tag tcc cca cct tt-3' | λgt10 |
| 8 | λ10-R | 24 mer | 5'-ctt atg agt att tct tcc agg gta-3' | λgt10 |
| 9 | SP6 | 24 mer | 5'-cat acg att tag gtg aca cta tag-3' | SP6 promoter |
| 10 | Z-1 | 19 mer | 5'-gcg tta ccc aac tta atc g-3' | lacZ gene |
| 11 | Z-2 | 18 mer | 5'-tgt gag cga gta aca acc-3' | lacZ gene |
| 12 | CAT-2 | 18 mer | 5'-act ggt gaa act cac cca-3' | CAT gene |
| 13 | CAT-3 | 21 mer | 5'-cag tca gtt gct caa tgt acc-3' | CAT gene |
| 14 | neo-F | 20 mer | 5'-aga ggc tat tcg gct atg ac-3' | neo gene |
| 15 | neo-R | 20 mer | 5'-cac cat gat att cgg caa gc-3' | neo gene |
| 16 | mG3PDH-1 | 19 mer | 5'-gga aag ctg tgg cgt gat g-3' | mouse G3PDH gene |
| 17 | mG3PDH-2 | 20 mer | 5'-ctg ttg ctg tag ccg tat tc-3' | mouse G3PDH gene |
| 18 | Cre-1 | 20 mer | 5'-aca tgt tca ggg atc gcc ag-3' | Cre gene |
| 19 | Cre-2 | 21 mer | 5'-taa cca gtg aaa cag cat tgc-3' | Cre gene |
| 20 | Cre-3 | 20 mer | 5'-aat gct tct gtc cgt ttg cc-3' | Cre gene |
| 21 | Cre-4 | 20 mer | 5'-cta cac cag aga cgg aaa tc-3' | Cre gene |
| 22 | ori-1 | 20 mer | 5'-aga ggc ggt ttg cgt att gg-3' | pUC origin |
| 23 | ori-2 | 20 mer | 5'-cag tgg cga taa gtc gtg tc-3' | pUC origin |
| 24 | BG-1 | 21 mer | 5'-atc cag gtt aca agg cag ctc-3' | mouse beta-globin gene |
| 25 | BG-2 | 21 mer | 5'-tta ccc tca ctt cca tcc ctc-3' | mouse beta-globin gene |
| 26 | BG-3 | 21 mer | 5'-aga aca gag tga gag gga gca-3' | mouse beta-globin gene |
| 27 | loxP-B | 22 mer | 5'-gat ccg gaa ccc tta ata taa c-3' | loxP site |
| 28 | loxP-P | 20 mer | 5'-agg tcg agg gac cta ata ac-3' | loxP site |
| 29 | lox66-B | 21 mer | 5'-atc cgg aac cct taa tta ccg-3' | mutant lox site |
| 30 | lox71-P | 19 mer | 5'-ggt cga ggg acc tat acc g-3' | mutant lox site |
| 31 | En2-SA | 20 mer | 5'-atg aca acc agg tcc cag gt-3' | En2 gene splice accepter |
| 32 | 34W-1 | 34 mer | 5'-ctg ttt gct acc taa aga agg cct ttt ttc tgg t-3' | 34 bp size marker |
| 33 | 34W-2 | 34 mer | 5'-acc aga aaa aag gcc ttc ttt agg tag caa aca g-3' | 34 bp size marker |
| 34 | HBx-1 | 20 mer | 5'-tct acc gtc ccc ttc ttc at-3' | HBV HBx gene |
| 35 | HBx-2 | 21 mer | 5'-caa ttt atg cct aca gcc tcc-3' | HBV HBx gene |
| 36 | Ori-3 | 21 mer | 5'-cac aga atc agg gga taa cgc-3' | pUC origin |
| 37 | EL-9 | 20 mer | 5'-cct aga cgt tgt aag aag gc-3' | mouse E-selectin |
| 38 | EL-11 | 20 mer | 5'-gat tgg aca ctc aat gga tc-3' | mouse E-selectin |
| 39 | bsr-1 | 21 mer | 5'-ctt ctc tgt cgc tac ttc tac-3' | bsr gene |
| 40 | bsr-2 | 20 mer | 5'-gca gaa atc gga gga aga ag-3' | bsr gene |
| 41 | bsr-3 | 20 mer | 5'-caa ctc cct aca cat acc ac-3' | bsr gene |
| 42 | AC-1 | 19 mer | 5'-tcg gct tct ggc gtg tga c-3' | AC promoter |
| 43 | AG-1 | 20 mer | 5'-ggt tgt tgt gct gtc tca tc-3' | AG promoter |
| 44 | AG-2 | 20 mer | 5'-ctg cta acc atg ttc atg cc-3' | AG promoter |
| 45 | GFP-1 | 20 mer | 5'-tac ggc aag ctg acc ctg aa-3' | GFP gene |
| 46 | GFP-2 | 20 mer | 5'-tgt gat cgc gct tct cgt tg-3' | GFP gene |
| 47 | AAT-7 | 18 mer | 5'-gtg tct aag gct gac tgg-3' | AAT promoter |
| 48 | AAT-21 | 20 mer | 5'-cct ggt ctg aat gtg tgt gc-3' | AAT promoter |
| 49 | AAT-31 | 18 mer | 5'-tgt ccc agg tca gtg gtg-3' | AAT promoter |
| 50 | PAC-1 | 20 mer | 5'-tcc atg acc gag tac aag cc-3' | PAC gene |
| 51 | PAC-2 | 20 mer | 5'-ggc ctt cca tct gtt gct gc-3' | PAC gene |
| 52 | bsd-1 | 19 mer | 5'-ggt ctc cca tgc ctt tgt c-3' | bsd gene |
| 53 | bsd-2 | 19 mer | 5'-cac ata acc aga ggg cag c-3' | bsd gene |
| 54 | AG-3 | 18 mer | 5'-gat cct gag aac ttc agg-3' | AG promoter |
| 55 | AG-4 | 19 mer | 5'-acc acc ttc tga tag gca g-3' | AG promoter |
| 56 | AG-5 | 19 mer | 5'-tag gca gcc tgc acc tga g-3' | AG promoter |
| 57 | AG-6 | 20 mer | 5'-cag aag tca gat gct caa gg-3' | AG promoter |
| 58 | RGpA-1 | 20 mer | 5'-tca tag ctg tcc ctc ttc tc-3' | |
| 59 | RGpA-2 | 21 mer | 5'-ctg ctg tcc att cct tat tcc-3' | |
| 60 | AG3AS | 19 mer | 5'-cct gaa gtt ctc agg atc c-3' | |
| 61 | pSP72-1 | 19 mer | 5'-ggg ctg gct tta cta tgc g-3' | pSP72 |
| 62 | pSP72-2 | 20mer | 5'-ata gtt aag cca gcc ccg ac-3' | pSP72 |
| 63 | pSP72-3 | 20 mer | 5'-ata agg gcg aca cgg aaa tg-3' | pSP72 |
| 64 | pSP72-4 | 20mer | 5'-agt aag ttg gcc gca gtg tt-3' | pSP72 |
| 65 | pSP72-5 | 21mer | 5'-tca cgt taa ggg att ttg gtc-3' | pSP72 |
| 66 | pSP72-6 | 20 mer | 5'-tgt agg tat ctc agt tcg gt-3' | pSP72 |
| 67 | AAT-8 | 20 mer | 5'-acc tgg gac agt cta gaa ct-3' | |
| 68 | AAT-9 | 20 mer | 5'-ttg ccc ctt ctg tca ttc ac-3' | |
| 69 | pUpA | 20mer | 5'-tat tcc ttt gcc ctc gga cg-3' | |
| 70 | pUpA-S | 24mer | 5'-cga gaa att gat gat cta tta aac-3' | |
| 71 | SA-1 | 24 mer | 5'-tcc tct ttg tta ggg ttc ttc ttc-3' | EN2 gene splice acceptor |
| 72 | SA-2 | 19 mer | 5'-gcc ctt tct cct cca tga c-3' | EN2 gene splice acceptor |
| 73 | SA-3 | 20 mer | 5'-gca aac gag gcc aga aag at-3' | EN2 gene splice acceptor |
| 74 | SA-4 | 20mer | 5'-tta cat agg aag ggg acg ga-3' | EN2 gene splice acceptor |
| 75 | SA-5 | 21mer | 5'-ggt cac ttt atg ttc ttg ccc-3' | EN2 gene splice acceptor |
| 76 | SA-6 | 20mer | 5'-agg tct caa gtt tta gcc gg-3' | EN2 gene splice acceptor |
| 77 | SA-7 | 20mer | 5'-atg tcc tct ggt gct caa ag-3' | EN2 gene splice acceptor |
| 78 | SA-8 | 20 mer | 5'-ata gga gtt tgc gga ggt tg-3' | EN2 gene splice acceptor |
| 79 | SA-9 | 28 mer | 5'-ata gtc gac ttc ttc ttt ggt ttt cgg g-3' | EN2 gene splice acceptor |
| 80 | SA-10 | 19 mer | 5'-agc agt gaa ggc tgt gcg a-3' | EN2 gene splice acceptor |
| 81 | SA-11 | 20 mer | 5'-ccc gaa aac caa aga aga ag-3' | EN2 gene splice acceptor |
| 82 | LZUS-3 | 19 mer | 5'-cgc atc gta acc gtg cat c-3' | |
| 83 | STOP-1 | 29 mer | 5'-taa ctg agt aga cta gtc tag aat tcg gc-3' | |
| 84 | STOP-2 | 33 mer | 5'-tcg agc cga att cta gac tag tct act cag tta-3' | |
| 85 | polyT-Xho | 24 mer | 5'-ccc ctc gag ttt ttt ttt ttt ttt-3' | |
| 86 | polyT20-Xho | 29 mer | 5'-ccc ctc gag ttt ttt ttt ttt ttt ttt tt-3' | |
| 87 | VC-11 | 20 mer | 5'-caa cga tct ctg tac atc cc-3' | |
| 88 | VC-12 | 20 mer | 5'-aga ggc tgt aca ctc tgc ct-3' | |
| 89 | VC-13 | 21 mer | 5'-ccc ttg aac aga tca atc tcc-3' | |
| 90 | VC-15 | 20 mer | 5'-gca gag act tga aat gcc tg-3' | |
| 91 | Amp-S | 20mer | 5'-tac agg cat cgt ggt gtc ac-3' | |
| 92 | Amp-As | 20mer | 5'-aaa tgt gcg cgg aac ccc ta-3' | |
| 93 | Primer3 | 24mer | 5'-ccc gtg gtg gtg ttc gac ttt gcc-3' | |
| 94 | Primer4 | 24 mer | 5'-gca caa aga tgg agt ccg tgt ccc-3' | |
| 95 | 154-1 | 23 mer | 5'-ttc ttc gtc aag gct cac gtg cg-3' | |
| 96 | 154-2 | 25 mer | 5'-ccg agt tac aca cga cct tga tgg c-3' | |
| 97 | 297-1 | 26mer | 5'-aag gtc gtg tgt aac tcg gtg tac gg-3' | |
| 98 | 297-2 | 23 mer | 5'-gat gat gcg cat gga ata ggg cc-3' | |
| 99 | hyg-pA | 20mer | 5'-tgc ctt cct tga ccc tgg aa-3' | |
| 100 | AUAP | 20 mer | 5'-ggc cac gcg tcg act agt ac-3' | |
| 101 | Flp-2 | 20 mer | 5'-tcg gaa gaa gca gat aag gg-3' | |
| 102 | Flp-5 | 20mer | 5'-atc cta ccc ctt gcg cta aa-3' | |
| 103 | Z-5 | 20 mer | 5'-acg gcg gat tga ccg taa tg-3' | lacZ gene |