At the Gene Technology Center, based on the Kumamoto University Gene Technology Center Internal Regulations, part of the expenses related to facility use will be borne by the user as a usage fee. Every year, usage from October 1st of the previous year to September 30th of the current year is calculated, the applicable courses are notified, and procedures for transferring costs are begun. See the Usage Fee Schedule for what incurs fees and the fee totals.
Usage Fee Schedule [from October 1st, 2020]
| （Ａ）Equipment usage fee |
| [1] Copy machine (Room 606) Black and white: 10 yen per copy, Color: 60 yen per copy |
100 yen per X-ray film |
| [3] Confocal Laser Scanning Microscope (FV3000, Olympus, Room 507) Usage fee: 500 yen per hour |
| [4] Capillary sequencer (ABI PRISM 310, Applied Biosystems, Room 502) Long capillary: 1 sample 350 yen |
| [5] Electrophoresis Image Processor (Printgraph, ATTO, Room 501) 10 yen per print |
| [6] Carbon dioxide incubator (CO2 incubator, Panasonic, Room 514) One month registration fee: 500 yen per person |
| [7] Flow cytometer (BD FACS Verse, BD Biosciences, Room 502) Usage fee: 300 yen per hour |
| [8] Desktop type ultracentrifuge (OptimaTLX, BECKMAN COULTER, Room 514) Usage fee: 1,000 yen per usage |
| [9] Real-time PCR (7500 System, Applied Biosystems, Room 502) Usage fee: 1,000 yen per usage |
| [10] Ink jet printer 30 yen per print |
| [11] Various PCR machines (Room 502) Usage fee: 100 yen per use |
| [12] Cryostat (CM3050S, Leica, Room 508) Usage fee: 1,000 yen per use |
| [13] Gene induction apparatus (Gene Pulser II System D, Bio-Rad, Room 502) Usage fee: 100 yen per use |
| [14] Inverted Research Microscope (IX73, Olympus, Room 507) Usage fee: 100 yen per hour |
| [15] All-in-one fluorescence microscope (Biozero BZ-8000, Keyence, Room 514) Usage fee: 100 yen per hour |
| [16] Microplate reader (iMark, Bio-Rad, Room 502) 100 yen per microplate |
| [17] Multi-microplate reader (SH-9000 Lab, Corona, Room 502) 200 yen per microplate |
| [18] GeneChip Analysis System (Affymetrix, Room 502) 1,000 yen per use |
| [19] Electroporation (Neon Transfection System, Invitrogen, Room 514) 100 yen per use |
| [20] Ultrasonic Homogenizer (SONIFIER Model 250, BRANSON, Room 502) 100 yen per use |
| [21] Incubator shaker (Innova 42, New Brunswick, Room 501) 200 yen per use |
| [22] Paraffin-embedded block preparation apparatus (TEC-IV, Tissue-Tek, Room 508) 500 yen per use |
| [23] Biochemical Analyzer (DryChem, Fuji Film, Room 503) Electrolytes, auto – 100 yen per slide Electrolytes, manual – 50 yen per slide Non-electrolytes, auto – 100 yen per slide Non-electrolytes, manual – 50 yen per slide |
| [24] Rotary microtome (RM 2245, Leica, Room 508) 100 yen per use |
| （Ｂ）Computer Related |
| [1] GENETYX (annual registration fee) Two types: GENETYX for Mac, GENETYX for Win 20,000 yen for the first client computer, 1,000 yen per computer for the second and subsequent computers |
| （Ｃ）Reagents and Expendable items |
| [1] Primer list (for PCR) *See details |
| [2] Disposable products (Centrifuge tubes, tips, filters, etc.) *See details |
| [3] Other expendable items |
| （Ｄ）Space usage |
| [1] Refrigerated showcase (4°C.) (Room 501, 502, 514) Usage fee per month per space: 600 yen |
| [2] Freezer (-25 °C.) (Room 501, 514) Upper shelf (A to F) Usage fee per month per space: 400 yen Lower shelf (G to J) Usage fee per month per space: 600 yen |
| [3] Deep Freezer (-80 °C) (Room 501, 503, 508, 509) Usage fee per month per rack: 1,200 yen, per drawer: 300 yen |
| [4] Large liquid nitrogen tank (for cultured cells, Room 509) Usage fee per month per box: 800 yen |
| [5] Liquid nitrogen tank (for cultured cells, Room 514) Usage fee per month per area: 300 yen |
| [6] Low temperature room (Room 516) *Currently not available Usage fee per month per area: 500 yen |
| [7] Storage shelf and laboratory table drawer Usage fee per month per space: 250 yen |
| [8] Dedicated laboratory table (Room 501, 514) Usage fee per month per space: 3,000 yen |
| （Ｅ）Contracted work |
| [1] Plasmid stock (GTC P-Stock) One year storage fee: 1 sample 2,000 yen Shipping agency fee: 1 item 1,000 yen |
| [2] Sequence contracting Contracting fee: Sequence reaction and electrophoresis 1,200 yen per sample Electrophoresis only : 500 yen per sample |
