Gene Technology Center

プライマーリスト(PCR用)

T3 や T7 などよく使われるプライマーを大量合成し、小分けしたものです。濃度100ピコモル/マイクロリットルで、100マイクロリットルずつ分注してあります(Total 10 nmol)。シーケンス反応に使用できるかどうかのチェックは行っていません。1本700円です。利用者負担金と同様に年1回集計します。購入を希望する方は、事務室でお申し込みください。

No. Name Size Sequence Note
1 T3 21 mer 5′-att aac cct cac taa agg gaa-3′ pBluescript II
2 T7 20 mer 5′-taa tac gac tca cta tag gg-3′ pBluescript II
3 M13-F 20 mer 5′-gtt gta aaa cga cgg cca gt-3′ M13 or pUC
4 M13-R 21 mer 5′-agg aaa cag cta tga cca tga-3′ M13 or pUC
5 λ11-F 24 mer 5′-ggt ggc gac gac tcc tgg agc ccg-3′ λgt11
6 λ11-R 24 mer 5′-ttg aca cca gac caa ctg gta atg-3′ λgt11
7 λ10-F 20 mer 5′-gct ggg tag tcc cca cct tt-3′ λgt10
8 λ10-R 24 mer 5′-ctt atg agt att tct tcc agg gta-3′ λgt10
9 SP6 24 mer 5′-cat acg att tag gtg aca cta tag-3′ SP6 promoter
10 Z-1 19 mer 5′-gcg tta ccc aac tta atc g-3′ lacZ gene
11 Z-2 20 mer 5′-tgt gag cga gta aca acc cg-3′ lacZ gene
12 CAT-2 18 mer 5′-act ggt gaa act cac cca-3′ chloramphenicol acetyltransferase (CAT) gene
13 CAT-3 21 mer 5′-cag tca gtt gct caa tgt acc-3′ chloramphenicol acetyltransferase (CAT) gene
14 neo-F 20 mer 5′-aga ggc tat tcg gct atg ac-3′ neomycin resistance gene [GenBank Accession:U43611]
15 neo-R 20 mer 5′-cac cat gat att cgg caa gc-3′ neomycin resistance gene [GenBank Accession:U43611]
16 mG3PDH-1 19 mer 5′-gga aag ctg tgg cgt gat g-3′ mouse G3PDH gene [GenBank Accession:M32599]
17 mG3PDH-2 20 mer 5′-ctg ttg ctg tag ccg tat tc-3′ mouse G3PDH gene [GenBank Accession:M32599]
18 Cre-1 20 mer 5′-aca tgt tca ggg atc gcc ag-3′ Cre gene
19 Cre-2 21 mer 5′-taa cca gtg aaa cag cat tgc-3′ Cre gene
20 Cre-3 20 mer 5′-aat gct tct gtc cgt ttg cc-3′ Cre gene
21 Cre-4 20 mer 5′-cta cac cag aga cgg aaa tc-3′ Cre gene
22 ori-1 20 mer 5′-aga ggc ggt ttg cgt att gg-3′ pUC origin
23 ori-2 20 mer 5′-cag tgg cga taa gtc gtg tc-3′ pUC origin
24 BG-1 21 mer 5′-atc cag gtt aca agg cag ctc-3′ mouse beta-globin gene (intron)
25 BG-2 21 mer 5′-tta ccc tca ctt cca tcc ctc-3′ mouse beta-globin gene (intron)
26 BG-3 21 mer 5′-aga aca gag tga gag gga gca-3′ mouse beta-globin gene (intron)
27 loxP-B 22 mer 5′-gat ccg gaa ccc tta ata taa c-3′ loxP site
28 loxP-P 20 mer 5′-agg tcg agg gac cta ata ac-3′ loxP site
29 lox66-B 21 mer 5′-atc cgg aac cct taa tta ccg-3′ mutant lox site
30 lox71-P 19 mer 5′-ggt cga ggg acc tat acc g-3′ mutant lox site
31 En2-SA 20 mer 5′-atg aca acc agg tcc cag gt-3′ En2 gene splice accepter
32 34W-1 34 mer 5′-ctg ttt gct acc taa aga agg cct ttt ttc tgg t-3′ 34 bp size marker
33 34W-2 34 mer 5′-acc aga aaa aag gcc ttc ttt agg tag caa aca g-3′ 34 bp size marker
34 HBx-1 20 mer 5′-tct acc gtc ccc ttc ttc at-3′ HBV HBx gene
35 HBx-2 21 mer 5′-caa ttt atg cct aca gcc tcc-3′ HBV HBx gene
36 Ori-3 21 mer 5′-cac aga atc agg gga taa cgc-3′ pUC origin
37 EL-9 20 mer 5′-cct aga cgt tgt aag aag gc-3′ mouse E-selectin
38 EL-11 20 mer 5′-gat tgg aca ctc aat gga tc-3′ mouse E-selectin
39 bsr-1 21 mer 5′-ctt ctc tgt cgc tac ttc tac-3′ blasticidin S-resistance gene (bsr)
40 bsr-2 20 mer 5′-gca gaa atc gga gga aga ag-3′ blasticidin S-resistance gene (bsr)
41 bsr-3 20 mer 5′-caa ctc cct aca cat acc ac-3′ blasticidin S-resistance gene (bsr)
42 AC-1 19 mer 5′-tcg gct tct ggc gtg tga c-3′ AC promoter
43 AG-1 20 mer 5′-ggt tgt tgt gct gtc tca tc-3′ rabbit beta-globin partial genomic DNA (intron/polyA signal)
44 AG-2 20 mer 5′-ctg cta acc atg ttc atg cc-3′ rabbit beta-globin partial genomic DNA (intron/polyA signal)
45 GFP-1 20 mer 5′-tac ggc aag ctg acc ctg aa-3′ GFP gene
46 GFP-2 20 mer 5′-tgt gat cgc gct tct cgt tg-3′ GFP gene
47 AAT-7 18 mer 5′-gtg tct aag gct gac tgg-3′ human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257]
48 AAT-21 20 mer 5′-cct ggt ctg aat gtg tgt gc-3′ human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257]
49 AAT-31 18 mer 5′-tgt ccc agg tca gtg gtg-3′ human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257]
50 PAC-1 20 mer 5′-tcc atg acc gag tac aag cc-3′ puromycin N-acetyltransferase (pac) gene
51 PAC-2 20 mer 5′-ggc ctt cca tct gtt gct gc-3′ puromycin N-acetyltransferase (pac) gene
52 bsd-1 19 mer 5′-ggt ctc cca tgc ctt tgt c-3′ blasticidin S-resistance gene (bsr)
53 bsd-2 19 mer 5′-cac ata acc aga ggg cag c-3′ blasticidin S-resistance gene (bsr)
54 AG-3 18 mer 5′-gat cct gag aac ttc agg-3′ rabbit beta-globin partial genomic DNA (intron/polyA signal)
55 AG-4 19 mer 5′-acc acc ttc tga tag gca g-3′ rabbit beta-globin partial genomic DNA (intron/polyA signal)
56 AG-5 19 mer 5′-tag gca gcc tgc acc tga g-3′ rabbit beta-globin partial genomic DNA (intron/polyA signal)
57 AG-6 20 mer 5′-cag aag tca gat gct caa gg-3′ rabbit beta-globin partial genomic DNA (intron/polyA signal)
58 RGpA-1 20 mer 5′-tca tag ctg tcc ctc ttc tc-3′ rabbit beta-globin partial genomic DNA (intron/polyA signal)
59 RGpA-2 21 mer 5′-ctg ctg tcc att cct tat tcc-3′ rabbit beta-globin partial genomic DNA (intron/polyA signal)
60 AG3AS 19 mer 5′-cct gaa gtt ctc agg atc c-3′ rabbit beta-globin partial genomic DNA (intron/polyA signal)
61 pSP72-1 19 mer 5′-ggg ctg gct tta cta tgc g-3′ pSP72
62 pSP72-2 20mer 5′-ata gtt aag cca gcc ccg ac-3′ pSP72
63 pSP72-3 20 mer 5′-ata agg gcg aca cgg aaa tg-3′ pSP72
64 pSP72-4 20mer 5′-agt aag ttg gcc gca gtg tt-3′ pSP72
65 pSP72-5 21mer 5′-tca cgt taa ggg att ttg gtc-3′ pSP72
66 pSP72-6 20 mer 5′-tgt agg tat ctc agt tcg gt-3′ pSP72
67 AAT-8 20 mer 5′-acc tgg gac agt cta gaa ct-3′ human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257]
68 AAT-9 20 mer 5′-ttg ccc ctt ctg tca ttc ac-3′ human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257]
69 pUpA 20mer 5′-tat tcc ttt gcc ctc gga cg-3′ mouse PGK gene polyadenylation signal
70 pUpA-S 24mer 5′-gca gaa att gat gat cta tta aac-3′ mouse PGK gene polyadenylation signal
71 SA-1 24 mer 5′-tcc tct ttg tta ggg ttc ttc ttc-3′ mouse Engraled-2 (En2) gene splice acceptor
72 SA-2 19 mer 5′-gcc ctt tct cct cca tga c-3′ mouse Engraled-2 (En2) gene splice acceptor
73 SA-3 20 mer 5′-gca aac gag gcc aga aag at-3′ mouse Engraled-2 (En2) gene splice acceptor
74 SA-4 20mer 5′-tta cat agg aag ggg acg ga-3′ EN2 gene splice acceptor ※not work!
75 SA-5 21mer 5′-ggt cac ttt atg ttc ttg ccc-3′ mouse Engrailed-2 (En2) gene splice acceptor
76 SA-6 20mer 5′-agg tct caa gtt tta gcc gg-3′ mouse Engrailed-2 (En2) gene splice acceptor
77 SA-7 20mer 5′-atg tcc tct ggt gct caa ag-3′ mouse Engrailed-2 (En2) gene splice acceptor
78 SA-8 20 mer 5′-ata gga gtt tgc gga ggt tg-3′ mouse Engrailed-2 (En2) gene splice acceptor
79 SA-9 28 mer 5′-ata gtc gac ttc ttc ttt ggt ttt cgg g-3′ mouse Engrailed-2 (En2) gene splice acceptor
80 SA-10 19 mer 5′-agc agt gaa ggc tgt gcg a-3′ mouse Engrailed-2 (En2) gene splice acceptor
81 SA-11 20 mer 5′-ccc gaa aac caa aga aga ag-3′ mouse Engrailed-2 (En2) gene splice acceptor
82 LZUS-3 19 mer 5′-cgc atc gta acc gtg cat c-3′ lacZ gene
83 STOP-1 29 mer 5′-taa ctg agt aga cta gtc tag aat tcg gc-3′ universal terminator
84 STOP-2 33 mer 5′-tcg agc cga att cta gac tag tct act cag tta-3′ universal terminator
85 polyT-Xho 24 mer 5′-ccc ctc gag ttt ttt ttt ttt ttt-3′ polyT(15) + XhoI site
86 polyT20-Xho 29 mer 5′-ccc ctc gag ttt ttt ttt ttt ttt ttt tt-3′ polyT(20) + XhoI site
87 VC-11 20mer 5′-caa cga tct ctg tac atc cc-3′ vascular cell adhesion molecule (VCAM)
88 VC-12 20mer 5′-aga ggc tgt aca ctc tgc ct-3′ vascular cell adhesion molecule (VCAM)
89 VC-13 21mer 5′-ccc ttg aac aga tca atc tcc-3′ vascular cell adhesion molecule (VCAM)
90 VC-15 20mer 5′-gca gag act tga aat gcc tg-3′ vascular cell adhesion molecule (VCAM)
91 Amp-S 20mer 5′-tac agg cat cgt ggt gtc ac-3′ ampicillin resistance gene
92 Amp-AS 20mer 5′-aaa tgt gcg cgg aac ccc ta-3′ ampicillin resistance gene
93 Primer3 24mer 5′-ccc gtg gtg gtg ttc gac ttt gcc-3′ HSV
94 Primer4 24mer 5′-gca caa aga tgg agt ccg tgt ccc-3′ HSV
95 154-1 23mer 5′-ttc ttc gtc aag gct cac gtg cg-3′ HSV
96 154-2 25mer 5′-ccg agt tac aca cga cct tga tgg c-3′ HSV
97 297-1 26mer 5′-aag gtc gtg tgt aac tcg gtg tac gg-3′ HSV
98 297-2 23mer 5′-gat gat gcg cat gga ata ggg cc-3′ HSV
99 hyg-pA 20mer 5′-tgc ctt cct tga ccc tgg aa-3′ pIRES1hyg
100 FRT-S 20mer 5′-gct tca aaa gcg ctc tga ag-3′ FRT
101 AUAP 20mer 5′-ggc cac gcg tcg act agt ac-3′ abridged universal amplification primer for 5′-RACE
102 Flp-2 20mer 5′-tcg gaa gaa gca gat aag gg-3′ Flp gene
103 Flp-5 20mer 5′-atc cta ccc ctt gcg cta aa-3′ Flp gene
104 Z-5 20mer 5′-acg gcg gat tga ccg taa tg-3′ lacZ gene
105 PGKpA 22mer 5′-aac ttccat ttt agt gga cat c-3′ pU-21T pA2
106 SA-12 20mer 5′-gga aac aaa ctt ggc ctc ac-3′ mouse Engraled-2 (En2) gene splice acceptor
107 SA-13 20mer 5′-cct gtt ggt ctg aaa ctc ag-3′ mouse Engraled-2 (En2) gene splice acceptor
108 Z-6 20mer 5′-gcg att aag ttg ggt aac gc-3′ lacZ gene
109 GFP-3 20mer 5′-acg aga agc gcg atc aca tg-3′ GFP gene
110 Z-3 19mer 5′-ggc ctc ttc gct att acg c-3′ lacZ gene
111 Z-7 20mer 5′-tct ggc ctt cct gta gcc ag-3′ lacZ gene
112 Z-8 21mer 5′-gtt tta caa cgt cgt gac tgg-3′ lacZ gene
113 SV40pA-S 21mer 5′-gcc ata cca cat ttg tag agg-3′ pU-21T pA1
114 DT-A-1 20mer 5′-tac cac ggg act aaa cct gg-3′ DT-A gene
115 DT-A-2 20mer 5′-cgc tta acg ctt tcg cct gt-3′ DT-A gene
116 DT-A-3 21mer 5′-gtt gat gga gca agt cgg aac-3′ DT-A gene
117 DT-A-4 21mer 5′-cct tgt gta cca gat ttt ggc-3′ DT-A gene
118 DT-A-5 20mer 5′-gaa aac gtg gcc aag atg cg-3′ DT-A gene
119 PGKpro 20mer 5’-ttt gct cct tcg ctt tct gg-3’ PGK promoter
120 PGKpA-S 22mer 5′-aag atg tcc act aaa atg gaa g-3′ pU-21T pA2
121 GFP-1AS 20mer 5′-ttc agg gtc agc ttg ccg ta-3′ GFP gene
122 SA14AS 21mer 5′-cac atc cat gct gag gat gag-3′ mouse Engraled-2 (En2) gene splice acceptor
123 SA-ter-Kp 32mer 5′-atg gta cct aag cag tga agg cta tgc gag gc-3′ mouse Engraled-2 (En2) gene splice acceptor
124 WZ-14 21mer 5′-gct ctc tac agg tgg atc aag-3′ pU-21W
125 WZ-17 21mer 5′-gca gtt caa tca gct gct ttc-3′ pU-21W
126 SA-125 20mer ctc ctg gga act cta ctg cc-3′ mouse Engraled-2 (En2) gene splice acceptor
127 SA-125AS 20mer ggc agt aga gtt ccc agg ag mouse Engraled-2 (En2) gene splice acceptor
128 pSP72-4R 20mer 5′-aac act gcg gcc aac tta ct-3′ pSP72
129 LPBL 27mer 5′-gga tcc gga acc ctt aat ata act tcg-3′ mouse Engraled-2 (En2) gene splice acceptor
130 Sry-F 20mer 5′-tga ctg gga tgc agt agt tc-3′ mouse Sry gene
131 Sry-R 20mer 5′-tgt gct aga gag aaa ccc tg-3′ mouse Sry gene
132 pSP72-7 21mer 5′-tca agg atc tta ccg ctg ttg-3′ pSP72
133 pSP72-8 21mer 5′-gtg aaa acc tct gac aca tgc-3′ pSP72
134 3RACE-AP 30mer 5′-ggc cac gcg tcg act agt act ttt ttt ttt-3′ 3′-RACE
135 SA12-AS 20mer 5′-gtg agg cca agt ttg ttt cc-3′ mouse Engraled-2 (En2) gene splice acceptor
136 SA14 21mer 5′-ctc atc ctc agc atg gat gtg-3′ mouse Engraled-2 (En2) gene splice acceptor
137 SA8-AS 20mer 5′-caa cct ccg caa act cct at-3′ mouse Engraled-2 (En2) gene splice acceptor
138 iCre-1 20mer 5′-agg cct tct ctg aac aca cc-3′ iCre
139 iCre-2 21mer 5′-ttg gtc ctg cca atg tgg atc-3′ iCre
140 SA3AS 20mer 5′-atc ttt ctg gcc tcg ttt gc-3′ mouse Engraled-2 (En2) gene splice acceptor
141 SA3.5AS 23mer 5′-aag agt agg cta gcc agt caa gg-3′ mouse Engraled-2 (En2) gene splice acceptor
142 SA4.5AS 25mer 5′-ttc ctg tcc agg aga ggg acc tgg c-3′ mouse Engraled-2 (En2) gene splice acceptor
143 SA5AS 21mer 5′-ggg caa gaa cat aaa gtg acc-3′ mouse Engraled-2 (En2) gene splice acceptor
144 SA6AS 22mer 5′-ccg gct aaa act tga gac ctt c-3′ mouse Engraled-2 (En2) gene splice acceptor
145 SA7AS 23mer 5′-tct ttg agc acc aga gga cat cc-3′ mouse Engraled-2 (En2) gene splice acceptor
No. Name Size Sequence Note
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