201903,Kataoka

研究発表を行った学会；
2019 AMMRA & AMPC Meeting
2019年 2月20日〜21日（Melbourne, Australia）
タイトル；Motor protein MyosinX is a novel regulatory factor for longitudinal bone growth and trabecular bone microstructures via the normal endochondral ossification in the growth plate.
発表者；片岡　太郎氏
（熊本大学　生命資源研究・支援センター　疾患モデル分野）
要旨；
We have explored the trabecular bone structure-related genes by using mouse forward genetic approaches and revealed that the Myo10 is involved in the bone formation during the growth phase. Myo10 is unconventional myosin which is well known as the roles of filopodia formation. Myo10 is expressed in many mammalian cells, but little is known about its functions at the organismal level. In order to analyze Myo10 functions in vivo, we produced Myo10 knockout mice (Myo10-KO) using CRISPR-Cas9 system. Myo10-KO mice showed thinner trabecular thickness as compared to control mice at 10 weeks of age. This phenotype conflicts with the published report, in which Myo10 is essential for osteoclast differentiation and osteoclastic bone resorption in vitro. In addition, Myo10-KO exhibited shorter tibia than that of control mice. Interestingly, Myo10-KO have a thicker growth plate as compared with control mice at 2 weeks of age, but their growth plate thickness was markedly declined to the control level until the 6 weeks of age. It is possible that Myo10 is involved in the regulation of chondrocytic differentiation and/or proliferation at growth plate rather than the osteoclastic bone resorption in the organism.