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研究発表を行った学会;2017年度 生命科学系学会合同年次大会(ConBio2017)
タイトル;Roles of the Cdc48-cofactor complexes in regulation of mitochondrial morphology in yeast.
発表者;Abhijit Chowdhury氏
(熊本大学 発生医学研究所 分子細胞制御分野)
Mitochondria are dynamic organelles undergoing continuous and regulated fusion and fission. We have found that the cytosolic AAA chaperone Cdc48 is involved in the regulation of mitochondrial fusion and efficient turnover of mitochondrial outer membrane fusion factor Fzo1. Cdc48 generally cooperates with cofactor proteins, which are responsible for substrate recognition and processing. Among ~20 Cdc48 cofactors in the budding yeast Saccharomyces cerevisiae, deletion of UBX2 showed impaired turnover of Fzo1 without alteration of mitochondrial morphology. By contrast, UBP3 deletion caused fragmented and aggregated mitochondria without affecting Fzo1 turnover. Sucrose density gradient centrifugation reveled that the loss of UBX2 perturbed disassembly of Fzo1 oligomers and deletion of UBP3 did not markedly affect the Fzo1 oligomerization. These results indicated that two different Cdc48-cofactor complexes independently regulate mitochondrial fusion and Fzo1 turnover. We are currently focusing on the mechanisms how these cofactors work on mitochondrial surface.


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