201907Ahmad

研究発表を行った学会；Japanese Society Developmental Biologists, JSDB
2019年 5月14日〜17日（大阪）
タイトル； Tsukushi affects hippocampal neurogenesis in mouse brain.
発表者；Shah Adil Ishtiyaq Ahmad氏
（熊本大学　大学院生命科学研究部　神経分化分野）
要旨；
Tsukushi (TSK) is a small signaling molecule which is involved in various developmental events in different vertebrate organisms. TSK was first reported to be involved in the early development of Chicken by functioning as a BMP antagonist (Ohta et al. 2004). Several following studies reported the interaction of TSK with major signaling cascades like Wnt, TGF-, BMP, FGF and Notch; as it regulates various developmental processes (Ahmad et al. 2018). Hippocampal neurogenesis is regulated by multiple signaling pathways, like Wnt, BMP, Notch, Shh, GABA etc (Goncalves et al. 2016). Since TSK directly interacts with BMP, Wnt and Notch pathways, we decided to investigate the role of TSK in mouse hippocampal neurogenesis. At first, we checked the expression of TSK in mouse hippocampus at different developmental stages. TSK is strongly expressed at hippocampal regions during early developmental stages starting as early as embryonic day 18. Along with the development, TSK expression is reduced and limited to particular regions of brain. In early stages, TSK is expressed in all the hippocampal regions (DG, CA1, CA2 and CA3), while at postnatal day 30, the expression is restricted to only DG region. Following BrdU injection of wild type and TSK knockout (TSK KO) mice, the DG area of TSK KO mice showed higher number of BrdU incorporated cells. The increased rate of cell proliferation in TSK KO mice is also evident by the immunostaining for Ki67. Both these results suggest possible regulatory role of TSK in the maintenance of hippocampal neurogenic niche. We will do further analysis to clarify the role of TSK in hippocampal neurogenesis with special attention to its influence on different signaling cascades. We also want to investigate the role of TSK in behavior and memory formation.