202207Nakagawa

研究発表を行った学会；オリジナルデータ
タイトル； Efficient generation of electroporation-mediated genome-edited rat via in vitro fertilization using cryopreserved sperm.
発表者；中川　佳子氏
（熊本大学　生命資源研究・支援センター　資源開発分野、プラチナバイオ株式会社）
要旨；
Recently, we newly reported sperm cryopreservation and in vitro fertilization (IVF) protocols for rats (Scientific Reports 10:93 2020). Sperm cryopreservation technology is useful for the preservation of genetically modified rat strains. Using frozen-thawed sperm obtained from one male rat, many pups can be efficiently produced via multiple IVF and embryo transfer. In this study, we demonstrated efficient electroporation-mediated generation of genome-edited rats via IVF using frozen-thawed sperm. We created zygotes with a high fertilization rate, and genome-edited rats with a sufficient birth rate and a high mutation rate. Since we can obtain a huge number of frozen sperm from a male rat and can subsequently perform IVF multiple times using the frozen-thawed sperm, we believe that our protocol will achieve further improvements in the field of efficient generation of genome-edited rats.